Allen Cell Discussion Forum

Topic	Replies	Views	Activity
Modification of H&E Tissue Stain Education	0	504	August 26, 2021
Targeting transcriptionally silent gene Gene-editing Discussion	2	285	June 2, 2023
RNA sequences from layer 23 pyramidal neuron General sequencing	1	342	June 24, 2023
Can I use Glutamax versions of media and still get good differentiation to cardiac? hiPSC Culture Discussion	1	340	January 5, 2023
Is it possible to inactivate GFP after knockout of my target gene by a second round of CRISPR/Cas9 genome editing? Gene-editing Discussion	1	334	January 3, 2023
Have you used our resources in your work? We want to hear from you! General	0	454	September 22, 2021
Advice of Lipofectamine STEM protocol hiPSC Culture Discussion	1	306	December 4, 2024
Aicssegmentation not found in Jupyter, Classic Segmentation Other Discussions getting-started , segmenter	3	216	February 24, 2024
Code for counting individual cells in colonies hiPSC Culture Discussion	1	305	July 18, 2023
Defrosting in 100mm dish hiPSC Culture Discussion	1	298	August 16, 2023
Antibiotic resistance in WTC derived lines? hiPSC Culture Discussion	1	279	March 9, 2023
Image Reading Error Classic Segmenter Software & Code segmenter , segmentation	0	384	June 21, 2022
iPS cardiac differentiation with lactate hiPSC Culture Discussion	1	266	November 1, 2023
Follow up on earlier topic - GFR vs. hESC qualified matrigel hiPSC Culture Discussion	0	368	November 7, 2022
Cannot find image metadata on 'Generative Modeling with Conditional Autoencoders: Building an Integrated Cell' dataset Software & Code	1	259	October 5, 2023
Watershed import error napari allen segementer Software & Code napari-allencell-segmenter , napari , segmentation , segmenter	0	361	June 18, 2022
AICSImage not Defined, Classical Segmenter Software & Code segmentation , getting-started , segmenter	2	204	February 27, 2024
Cannot log in to careers account Other Discussions	1	233	September 4, 2023
Error in the source code of suggest_normalization_param Software & Code segmentation	0	322	June 21, 2022
sgRNA transfection Gene-editing Discussion	0	307	January 23, 2023
Evaluating morphology of hiPS colonies Other Discussions	1	187	July 25, 2024
Commercially available, Genetically Encoded Calcium Indicator stable iPSC-cell line? Other Discussions	1	184	February 9, 2024
Demethylating haldol affected genes Education	4	112	November 12, 2024
Background on sample Data Software & Code	1	177	December 4, 2023
Differences in z-score values for the same region, same donor and same probe Other Discussions	0	221	November 4, 2023
Cell morphology by Cell Types General cell-feature-explorer	0	203	November 21, 2023
FNet setup issues due to outdated dependencies – any updated version or Docker? Software & Code getting-started	5	82	March 25, 2026
Background to Data Other Discussions cell-feature-explorer	0	198	September 7, 2023
WTC-11 Genome Sequence in the UCSC Browser Other Discussions	3	97	October 8, 2025
Human mesenchymal stem cell culture Other Discussions	0	189	November 22, 2023
Single cell cluster naming General	1	133	November 27, 2024
(AICS-0074-026) MONO-ALLELIC mEGFP-TAGGED SOX hiPSCs Thawing/freezing question Other Discussions	2	107	March 20, 2025
Is there any timelapse microscopy data for fluorescently tagged hiPSCs available for download? Download	1	128	May 16, 2024
Found way around not well formed error and itkwidgets error, Classical Segmenter Other Discussions segmentation , getting-started , segmenter	0	171	March 8, 2024
Protocol for Parental Cell Line (GM25256) hiPSC Culture Discussion	1	118	February 17, 2025
Culturing hiPSC and Other Cell Lines in Same Lab Space hiPSC Culture Discussion	1	113	April 2, 2025
Hypothalmus tissues not included in the Allen Mouse Brain Common Coordinate Framework version 3 (CCFv3) Other Discussions	2	91	January 29, 2025
How to access cell lines Gene-editing Discussion	1	109	June 20, 2024
AICS-0126-068:WTC-mEGFP-CDH5 and AICS-0126-041:WTC-mEGFP-CDH5 hiPSC Culture Discussion	1	107	March 20, 2025
Drug perturbation pilot study metadata is missing Download	3	64	March 1, 2026
AICS-0037-172-MONO-ALLELIC mEGFP-TAGGED TNNI iPSCs RFP Signal hiPSC Culture Discussion	1	88	February 4, 2025
Cvapipe_analysis, cannot run test case (shape analysis on 300 cells) Software & Code bug-report	1	87	March 19, 2025
Drug perturbation single cell segmentation Other Discussions segmentation	1	86	July 2, 2024
Recreating gif style figure on Spherical Harmonics read me Other Discussions	1	82	June 26, 2025
How to keep cardiac differentiated cells from coming apart lifting from well hiPSC Culture Discussion	1	80	July 30, 2024
Issues with dCAS9-BFP line Other Discussions	1	73	February 19, 2026
Thaw, expand and maintain MONO-ALLELIC mEGFP-TAGGED SOX2 cells in E8+ flex medium (Invitrogen) on Vitronectin-N coated wells hiPSC Culture Discussion getting-started	1	66	July 30, 2025
Brain and body donation to Allen Institute to support specified research Other Discussions segmentation	1	62	November 13, 2025
Western Blot background GFP bands-- how did you reduce this in WTC-11? hiPSC Culture Discussion	1	56	April 29, 2026
Tips for reducing non-specific antibody binding in WTC-11 cells on coated glass hiPSC Culture Discussion	0	77	April 23, 2025