Allen Cell Discussion Forum

Topic	Replies	Views	Activity
Lentivirus transduction Gene-editing Discussion	1	284	July 6, 2023
Is it possible to inactivate GFP after knockout of my target gene by a second round of CRISPR/Cas9 genome editing? Gene-editing Discussion	1	284	January 3, 2023
Targeting transcriptionally silent gene Gene-editing Discussion	2	228	June 2, 2023
RNA sequences from layer 23 pyramidal neuron General sequencing	1	278	June 24, 2023
Facing Problems while working with AICSImage package for CZI files aiscimageio	4	175	April 11, 2024
Reason for Matrigel Concentration Recommended hiPSC Culture Discussion	1	267	August 16, 2023
Code for counting individual cells in colonies hiPSC Culture Discussion	1	245	July 18, 2023
Image Reading Error Classic Segmenter Software & Code segmenter , segmentation	0	345	June 21, 2022
Watershed import error napari allen segementer Software & Code napari-allencell-segmenter , napari , segmentation , segmenter	0	331	June 18, 2022
Antibiotic resistance in WTC derived lines? hiPSC Culture Discussion	1	232	March 9, 2023
Aicssegmentation not found in Jupyter, Classic Segmentation getting-started , segmenter	3	156	February 24, 2024
Follow up on earlier topic - GFR vs. hESC qualified matrigel hiPSC Culture Discussion	0	311	November 7, 2022
Defrosting in 100mm dish hiPSC Culture Discussion	1	213	August 16, 2023
Cannot find image metadata on 'Generative Modeling with Conditional Autoencoders: Building an Integrated Cell' dataset Software & Code	1	210	October 5, 2023
Error in the source code of suggest_normalization_param Software & Code segmentation	0	289	June 21, 2022
iPS cardiac differentiation with lactate hiPSC Culture Discussion	1	191	November 1, 2023
Cannot log in to careers account	1	191	September 4, 2023
sgRNA transfection Gene-editing Discussion	0	262	January 23, 2023
AICSImage not Defined, Classical Segmenter Software & Code segmentation , getting-started , segmenter	2	142	February 27, 2024
Differences in z-score values for the same region, same donor and same probe	0	191	November 4, 2023
Commercially available, Genetically Encoded Calcium Indicator stable iPSC-cell line?	1	135	February 9, 2024
Background on sample Data Software & Code	1	125	December 4, 2023
Background to Data cell-feature-explorer	0	171	September 7, 2023
Cell morphology by Cell Types General cell-feature-explorer	0	162	November 21, 2023
Human mesenchymal stem cell culture	0	146	November 22, 2023
Found way around not well formed error and itkwidgets error, Classical Segmenter segmentation , getting-started , segmenter	0	126	March 8, 2024
Is there any timelapse microscopy data for fluorescently tagged hiPSCs available for download? Download	1	81	May 16, 2024
Evaluating morphology of hiPS colonies	1	65	July 25, 2024
How to access cell lines Gene-editing Discussion	1	53	June 20, 2024
Demethylating haldol affected genes Education	4	32	November 12, 2024
(AICS-0074-026) MONO-ALLELIC mEGFP-TAGGED SOX hiPSCs Thawing/freezing question	2	31	March 20, 2025
Advice of Lipofectamine STEM protocol hiPSC Culture Discussion	1	35	December 4, 2024
Hypothalmus tissues not included in the Allen Mouse Brain Common Coordinate Framework version 3 (CCFv3)	2	27	January 29, 2025
Drug perturbation single cell segmentation segmentation	1	32	July 2, 2024
Single cell cluster naming General	1	30	November 27, 2024
AICS-0037-172-MONO-ALLELIC mEGFP-TAGGED TNNI iPSCs RFP Signal hiPSC Culture Discussion	1	27	February 4, 2025
Culturing hiPSC and Other Cell Lines in Same Lab Space hiPSC Culture Discussion	1	26	April 2, 2025
How to keep cardiac differentiated cells from coming apart lifting from well hiPSC Culture Discussion	1	26	July 30, 2024
AICS-0126-068:WTC-mEGFP-CDH5 and AICS-0126-041:WTC-mEGFP-CDH5 hiPSC Culture Discussion	1	24	March 20, 2025
Cvapipe_analysis, cannot run test case (shape analysis on 300 cells) Software & Code bug-report	1	23	March 19, 2025
Protocol for Parental Cell Line (GM25256) hiPSC Culture Discussion	1	23	February 17, 2025
Recreating gif style figure on Spherical Harmonics read me	1	20	June 26, 2025
Tips for reducing non-specific antibody binding in WTC-11 cells on coated glass hiPSC Culture Discussion	0	23	April 23, 2025