Allen Cell Discussion Forum

Topic	Replies	Views	Activity
Modification of H&E Tissue Stain Education	0	491	August 26, 2021
Targeting transcriptionally silent gene Gene-editing Discussion	2	262	June 2, 2023
Is it possible to inactivate GFP after knockout of my target gene by a second round of CRISPR/Cas9 genome editing? Gene-editing Discussion	1	317	January 3, 2023
RNA sequences from layer 23 pyramidal neuron General sequencing	1	316	June 24, 2023
Can I use Glutamax versions of media and still get good differentiation to cardiac? hiPSC Culture Discussion	1	316	January 5, 2023
Have you used our resources in your work? We want to hear from you! General	0	441	September 22, 2021
Code for counting individual cells in colonies hiPSC Culture Discussion	1	281	July 18, 2023
Aicssegmentation not found in Jupyter, Classic Segmentation getting-started , segmenter	3	193	February 24, 2024
Image Reading Error Classic Segmenter Software & Code segmenter , segmentation	0	373	June 21, 2022
Defrosting in 100mm dish hiPSC Culture Discussion	1	263	August 16, 2023
Antibiotic resistance in WTC derived lines? hiPSC Culture Discussion	1	260	March 9, 2023
Advice of Lipofectamine STEM protocol hiPSC Culture Discussion	1	258	December 4, 2024
Follow up on earlier topic - GFR vs. hESC qualified matrigel hiPSC Culture Discussion	0	350	November 7, 2022
Watershed import error napari allen segementer Software & Code napari-allencell-segmenter , napari , segmentation , segmenter	0	350	June 18, 2022
Cannot find image metadata on 'Generative Modeling with Conditional Autoencoders: Building an Integrated Cell' dataset Software & Code	1	240	October 5, 2023
iPS cardiac differentiation with lactate hiPSC Culture Discussion	1	234	November 1, 2023
AICSImage not Defined, Classical Segmenter Software & Code segmentation , getting-started , segmenter	2	181	February 27, 2024
Cannot log in to careers account	1	219	September 4, 2023
Error in the source code of suggest_normalization_param Software & Code segmentation	0	309	June 21, 2022
sgRNA transfection Gene-editing Discussion	0	295	January 23, 2023
Commercially available, Genetically Encoded Calcium Indicator stable iPSC-cell line?	1	166	February 9, 2024
Evaluating morphology of hiPS colonies	1	157	July 25, 2024
Differences in z-score values for the same region, same donor and same probe	0	212	November 4, 2023
Background on sample Data Software & Code	1	147	December 4, 2023
Cell morphology by Cell Types General cell-feature-explorer	0	189	November 21, 2023
Background to Data cell-feature-explorer	0	187	September 7, 2023
Human mesenchymal stem cell culture	0	171	November 22, 2023
Found way around not well formed error and itkwidgets error, Classical Segmenter segmentation , getting-started , segmenter	0	159	March 8, 2024
Is there any timelapse microscopy data for fluorescently tagged hiPSCs available for download? Download	1	111	May 16, 2024
Demethylating haldol affected genes Education	4	68	November 12, 2024
Single cell cluster naming General	1	92	November 27, 2024
WTC-11 Genome Sequence in the UCSC Browser	3	64	October 8, 2025
(AICS-0074-026) MONO-ALLELIC mEGFP-TAGGED SOX hiPSCs Thawing/freezing question	2	73	March 20, 2025
Protocol for Parental Cell Line (GM25256) hiPSC Culture Discussion	1	87	February 17, 2025
How to access cell lines Gene-editing Discussion	1	87	June 20, 2024
Culturing hiPSC and Other Cell Lines in Same Lab Space hiPSC Culture Discussion	1	86	April 2, 2025
Hypothalmus tissues not included in the Allen Mouse Brain Common Coordinate Framework version 3 (CCFv3)	2	68	January 29, 2025
AICS-0126-068:WTC-mEGFP-CDH5 and AICS-0126-041:WTC-mEGFP-CDH5 hiPSC Culture Discussion	1	83	March 20, 2025
Cvapipe_analysis, cannot run test case (shape analysis on 300 cells) Software & Code bug-report	1	73	March 19, 2025
FNet setup issues due to outdated dependencies – any updated version or Docker? Software & Code getting-started	5	41	March 25, 2026
Drug perturbation single cell segmentation segmentation	1	68	July 2, 2024
AICS-0037-172-MONO-ALLELIC mEGFP-TAGGED TNNI iPSCs RFP Signal hiPSC Culture Discussion	1	64	February 4, 2025
Recreating gif style figure on Spherical Harmonics read me	1	61	June 26, 2025
How to keep cardiac differentiated cells from coming apart lifting from well hiPSC Culture Discussion	1	61	July 30, 2024
Thaw, expand and maintain MONO-ALLELIC mEGFP-TAGGED SOX2 cells in E8+ flex medium (Invitrogen) on Vitronectin-N coated wells hiPSC Culture Discussion getting-started	1	50	July 30, 2025
Drug perturbation pilot study metadata is missing Download	3	35	March 1, 2026
Tips for reducing non-specific antibody binding in WTC-11 cells on coated glass hiPSC Culture Discussion	0	65	April 23, 2025
Issues with dCAS9-BFP line	1	44	February 19, 2026
Brain and body donation to Allen Institute to support specified research segmentation	1	40	November 13, 2025
Western Blot background GFP bands-- how did you reduce this in WTC-11? hiPSC Culture Discussion	1	27	April 29, 2026