Hi Allen team,
We are thinking to purchase a VE-cadherin tagged iPSC line and would like to ask you whether the monoallelic (AICS-0126-068) or biallelic (AICS-0126-041) one would be the most favorable to visualize the endothelial cells in 2D and 3D models.
Do you know if tagging of the VE-cadherin may affect its function, e.g. in terms of interaction with other proteins etc.?
Your feedback is highly appreciated!
Thank you very much!
Tatjana
Hi Tatjana,
Thanks for your question! The biallelic clone (AICS-0126-041) will have a brighter GFP signal and would be easier to visualize in 2D and 3D. We have not seen any differences in VE-cadherin localization between our tagged VE-cadherin lines and untagged lines as endothelial cells, but we have not comprehensively surveyed the interactions of the tagged VE-cadherin with other proteins.
Please let me know if there are any other questions I can help with!
Becky