Targeting transcriptionally silent gene

We have tried to target a transcriptionally silent gene with a reporter and antibiotic-resistant gene as a selection marker. We see confirmed integration of the cassette but no expression of the reporter upon differentiation. Is there a possibility that the antibiotic resistance gene is affecting the expression of the reporter?

Hi Pratiksha,

Thank you. We will need some more information about this experiment. Was selection performed? Could you maybe please share a map of the construct?


Thank you for the reply. Our selection marker is puromycin resistant gene. The selection was performed for 10 days. Please find the attached vector map. This segment was cloned into a pBSK vector backbone.