Hi team,
We differentiated the hiPSCs into cardiomyocytes and we observed that the RFP was observed but not GFP expression. Moreover, we didn’t observe the beating of the cardiomyocytes. Any suggestion would be highly appreciated.
Thanks
Maniteja
Hi Maniteja,
That is concerning and sounds like you or Coriell may have mixed up vials. We perform extensive quality control on all of our cell lines distributed in our Allen Institute Cell Collection. They had passed quality control and were successfully differentiated into cardiomyocytes and we observed beating. You can find that information on our cell catalog. Was this the only cell line you purchased from the collection? Any possibility they may have been mixed up with another cell line? What structure are you observing in the RFP channel as this line was not tagged with RFP. Also, do you have the order information from Coriell? Feel free to contact me directly at jacquelines@alleninstitute.org and we can try and troubleshoot what is going on.