hiPSC Culture Discussion

Topic	Replies	Views	Activity
About the hiPSC Culture Discussion category	0	788	September 24, 2018
Thaw, expand and maintain MONO-ALLELIC mEGFP-TAGGED SOX2 cells in E8+ flex medium (Invitrogen) on Vitronectin-N coated wells getting-started	1	17	July 30, 2025
Tips for reducing non-specific antibody binding in WTC-11 cells on coated glass	0	27	April 23, 2025
Culturing hiPSC and Other Cell Lines in Same Lab Space	1	40	April 2, 2025
AICS-0126-068:WTC-mEGFP-CDH5 and AICS-0126-041:WTC-mEGFP-CDH5	1	30	March 20, 2025
Protocol for Parental Cell Line (GM25256)	1	30	February 17, 2025
AICS-0037-172-MONO-ALLELIC mEGFP-TAGGED TNNI iPSCs RFP Signal	1	29	February 4, 2025
Advice of Lipofectamine STEM protocol	1	54	December 4, 2024
How to keep cardiac differentiated cells from coming apart lifting from well	1	27	July 30, 2024
Cardiomyocyte Differentiation Issue cardiac	12	1888	February 22, 2024
iPS cardiac differentiation with lactate	1	193	November 1, 2023
Reason for Matrigel Concentration Recommended	1	290	August 16, 2023
Defrosting in 100mm dish	1	214	August 16, 2023
Code for counting individual cells in colonies	1	246	July 18, 2023
Weekend free protocols for iPS maintenance	3	360	April 20, 2023
Antibiotic resistance in WTC derived lines?	1	234	March 9, 2023
Cryo-preserving in CryoStor?	2	301	January 20, 2023
Dilution of Matrigel : which media?	2	351	January 19, 2023
Can I use Glutamax versions of media and still get good differentiation to cardiac?	1	287	January 5, 2023
Conflicting Matrigel instructions	1	355	November 10, 2022
Matrigel vs geltrex	3	1000	November 10, 2022
Follow up on earlier topic - GFR vs. hESC qualified matrigel	0	313	November 7, 2022
Types of Matrigel- GFR? hESC qualified? matrigel	1	1827	November 4, 2022
Choice of lines for monitoring cardiac differentiation cardiac	1	316	September 22, 2022
mGFP-Tagged MYL2 cardiac	1	710	November 17, 2021
hiPSC differentiation	1	527	August 6, 2021
HLA type of WTC AKA WTC11	1	621	June 2, 2021
Culturing cells for imaging on glass	1	1095	July 7, 2020
When should I use ROCK inhibitor?	2	1946	December 7, 2019
Single cell vs colony passaging	1	1546	December 7, 2019