hiPSC Culture Discussion

Topic	Replies	Views	Activity
About the hiPSC Culture Discussion category	0	792	September 24, 2018
iPSC line AICS-0090-391 adipogenic differentiation	0	5	November 19, 2025
Thaw, expand and maintain MONO-ALLELIC mEGFP-TAGGED SOX2 cells in E8+ flex medium (Invitrogen) on Vitronectin-N coated wells getting-started	1	22	July 30, 2025
Tips for reducing non-specific antibody binding in WTC-11 cells on coated glass	0	31	April 23, 2025
Culturing hiPSC and Other Cell Lines in Same Lab Space	1	46	April 2, 2025
AICS-0126-068:WTC-mEGFP-CDH5 and AICS-0126-041:WTC-mEGFP-CDH5	1	35	March 20, 2025
Protocol for Parental Cell Line (GM25256)	1	36	February 17, 2025
AICS-0037-172-MONO-ALLELIC mEGFP-TAGGED TNNI iPSCs RFP Signal	1	33	February 4, 2025
Advice of Lipofectamine STEM protocol	1	78	December 4, 2024
How to keep cardiac differentiated cells from coming apart lifting from well	1	31	July 30, 2024
Cardiomyocyte Differentiation Issue cardiac	12	1911	February 22, 2024
iPS cardiac differentiation with lactate	1	200	November 1, 2023
Reason for Matrigel Concentration Recommended	1	306	August 16, 2023
Defrosting in 100mm dish	1	218	August 16, 2023
Code for counting individual cells in colonies	1	251	July 18, 2023
Weekend free protocols for iPS maintenance	3	373	April 20, 2023
Antibiotic resistance in WTC derived lines?	1	239	March 9, 2023
Cryo-preserving in CryoStor?	2	306	January 20, 2023
Dilution of Matrigel : which media?	2	358	January 19, 2023
Can I use Glutamax versions of media and still get good differentiation to cardiac?	1	290	January 5, 2023
Conflicting Matrigel instructions	1	367	November 10, 2022
Matrigel vs geltrex	3	1032	November 10, 2022
Follow up on earlier topic - GFR vs. hESC qualified matrigel	0	321	November 7, 2022
Types of Matrigel- GFR? hESC qualified? matrigel	1	1855	November 4, 2022
Choice of lines for monitoring cardiac differentiation cardiac	1	320	September 22, 2022
mGFP-Tagged MYL2 cardiac	1	713	November 17, 2021
hiPSC differentiation	1	533	August 6, 2021
HLA type of WTC AKA WTC11	1	627	June 2, 2021
Culturing cells for imaging on glass	1	1103	July 7, 2020
When should I use ROCK inhibitor?	2	2030	December 7, 2019