hiPSC Culture Discussion

Topic	Replies	Views	Activity
About the hiPSC Culture Discussion category	0	790	September 24, 2018
Thaw, expand and maintain MONO-ALLELIC mEGFP-TAGGED SOX2 cells in E8+ flex medium (Invitrogen) on Vitronectin-N coated wells getting-started	1	19	July 30, 2025
Tips for reducing non-specific antibody binding in WTC-11 cells on coated glass	0	29	April 23, 2025
Culturing hiPSC and Other Cell Lines in Same Lab Space	1	42	April 2, 2025
AICS-0126-068:WTC-mEGFP-CDH5 and AICS-0126-041:WTC-mEGFP-CDH5	1	32	March 20, 2025
Protocol for Parental Cell Line (GM25256)	1	32	February 17, 2025
AICS-0037-172-MONO-ALLELIC mEGFP-TAGGED TNNI iPSCs RFP Signal	1	32	February 4, 2025
Advice of Lipofectamine STEM protocol	1	65	December 4, 2024
How to keep cardiac differentiated cells from coming apart lifting from well	1	31	July 30, 2024
Cardiomyocyte Differentiation Issue cardiac	12	1900	February 22, 2024
iPS cardiac differentiation with lactate	1	199	November 1, 2023
Reason for Matrigel Concentration Recommended	1	295	August 16, 2023
Defrosting in 100mm dish	1	217	August 16, 2023
Code for counting individual cells in colonies	1	249	July 18, 2023
Weekend free protocols for iPS maintenance	3	368	April 20, 2023
Antibiotic resistance in WTC derived lines?	1	238	March 9, 2023
Cryo-preserving in CryoStor?	2	304	January 20, 2023
Dilution of Matrigel : which media?	2	354	January 19, 2023
Can I use Glutamax versions of media and still get good differentiation to cardiac?	1	290	January 5, 2023
Conflicting Matrigel instructions	1	361	November 10, 2022
Matrigel vs geltrex	3	1016	November 10, 2022
Follow up on earlier topic - GFR vs. hESC qualified matrigel	0	317	November 7, 2022
Types of Matrigel- GFR? hESC qualified? matrigel	1	1840	November 4, 2022
Choice of lines for monitoring cardiac differentiation cardiac	1	319	September 22, 2022
mGFP-Tagged MYL2 cardiac	1	712	November 17, 2021
hiPSC differentiation	1	529	August 6, 2021
HLA type of WTC AKA WTC11	1	625	June 2, 2021
Culturing cells for imaging on glass	1	1098	July 7, 2020
When should I use ROCK inhibitor?	2	1988	December 7, 2019
Single cell vs colony passaging	1	1557	December 7, 2019