Hello everyone, we recently ordered MONO-ALLELIC mEGFP-TAGGED SOX catalog ID AICS-0074-026.
We have technical questions regarding handling the cells right after thawing and when freezing:
- Can the cells be directly thawed in Essential 8 Flex medium and plated on vitronectin-coated plates rather than mTSER medium and Matrigel coating? If this is not possible, should we gradually transition from mTSER to Essential 8 Flex?
- According to the protocol, the freezing medium is mTeSR1 with 30% KSR, and 10% DMSO, so I assume the vial we ordered is in the same condition, could you confirm it?
- Since I plan to culture the cells in E8 flex, shall I freeze them, according to the above, using E8 Flex +30% KSR +10% DMSO?
Thank you very much in advance.