Can I use Glutamax versions of media and still get good differentiation to cardiac?

hiPSC Culture Discussion
1

Can I use RPMI with glutamax (also other versions with glutamine replaced with di peptide- Ala-Gln)
and still have good differentiation to cardiac?
I assume that mTesr1 Plus is similarly a replacement of glutamax for glutamine. Can this be used and still have good differentiation to cardiac?

2

Hi @hartw006,

We haven’t tried the cardiac differentiation protocol using RPMI with Glutamax or after culturing the hiPSCs in mTeSR1 Plus, so I cannot say for certain if the cardiac differentiation efficiency will be affected. I expect that the protocol would still work. If you give it a try, please let us know how it goes!

Thank you & best,
Becky