Pictures of Healthy vs Unhealthy hiPSC colonies

hiPSC Culture Discussion
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How should healthy iPSC colonies look? What characteristics define a bad colony?

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Example of a crater formation in hiPSC colonies, not healthy!

cratering3-01
cratering3-01.jpg1000×1000 736 KB

Healthier hiPSC colonies below, notice the smooth edges:

Healthy_AICS-803-01
Healthy_AICS-803-01.jpg1000×1000 694 KB

hiPSC colonies undergoing differentiation, look for triangular or spiky cells at edges:
differentiation_4x3-01

Underconfluence of hiPSC colonies:
underconfluent3-01

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Is there somewhere on the AICS website where I can find a collection of healthy and unhealthy colony images suitable for benchmarking a machine-learning based classifier?

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Hi @gregj, We do not have a set at this moment available on our website. However, maybe one of our scientists may be able to provide images. About how many images of healthy and unhealthy would you need for machine learning?

Thanks!