Can I passage cells on a Thursday or a Friday and do a double feed on a Saturday so that I can skip a feed on a Sunday?
Hi hperera! I routinely passage my cells on Thursday at a density where I can double feed on Friday and passage again on Monday (no feeding required on Saturday OR Sunday- hooray!).
For cell lines that do not require ROCK inhibitor when passaging (such as the parental WTC11s), I have also had success passaging on Friday directly into 6-well plates containing 4mL of medium per well and incubating until Monday.
Note: I use mTeSR Plus medium
Thank you for the information. What is the seeding density that you use for 6well plates? when passaging on Friday?
We typically seed 150K cells for 3 days, 75K for 4 days in 6 well plates. We find that growth is more dense at perimeter of the well and uneven at the center.