Hi, is it possible to replace matrigel with geltrex?
This is possible. In fact, some in our lab are doing this right now due to inconsistencies in matrigel lots and production issues.
I would recommend an, “adjustment period” of a couple passages before performing experiments and/or differentiations. It would also be a good idea to verify pluripotency via IF or Flow (I personally like the BD sciences mouse/human pluripotency kit).