Allen Cell Discussion Forum

Topic	Replies	Views	Activity
Western Blot background GFP bands-- how did you reduce this in WTC-11? hiPSC Culture Discussion	1	34	April 29, 2026
Endogenous base deletion ipsc generation Gene-editing Discussion	0	21	March 31, 2026
FNet setup issues due to outdated dependencies – any updated version or Docker? Software & Code getting-started	5	49	March 25, 2026
Finding Specific Track IDs in Vol-E Software & Code	1	27	March 23, 2026
Drug perturbation pilot study metadata is missing Download	3	40	March 1, 2026
Issues with dCAS9-BFP line	1	49	February 19, 2026
Pictures of Healthy vs Unhealthy hiPSC colonies hiPSC Culture Discussion	5	6453	January 20, 2026
iPSC line AICS-0090-391 adipogenic differentiation hiPSC Culture Discussion	0	31	November 19, 2025
Brain and body donation to Allen Institute to support specified research segmentation	1	43	November 13, 2025
WTC-11 Genome Sequence in the UCSC Browser	3	71	October 8, 2025
Thaw, expand and maintain MONO-ALLELIC mEGFP-TAGGED SOX2 cells in E8+ flex medium (Invitrogen) on Vitronectin-N coated wells hiPSC Culture Discussion getting-started	1	53	July 30, 2025
Recreating gif style figure on Spherical Harmonics read me	1	65	June 26, 2025
Tips for reducing non-specific antibody binding in WTC-11 cells on coated glass hiPSC Culture Discussion	0	67	April 23, 2025
Culturing hiPSC and Other Cell Lines in Same Lab Space hiPSC Culture Discussion	1	92	April 2, 2025
AICS-0126-068:WTC-mEGFP-CDH5 and AICS-0126-041:WTC-mEGFP-CDH5 hiPSC Culture Discussion	1	89	March 20, 2025
(AICS-0074-026) MONO-ALLELIC mEGFP-TAGGED SOX hiPSCs Thawing/freezing question	2	80	March 20, 2025
Cvapipe_analysis, cannot run test case (shape analysis on 300 cells) Software & Code bug-report	1	77	March 19, 2025
Protocol for Parental Cell Line (GM25256) hiPSC Culture Discussion	1	93	February 17, 2025
AICS-0037-172-MONO-ALLELIC mEGFP-TAGGED TNNI iPSCs RFP Signal hiPSC Culture Discussion	1	69	February 4, 2025
Hypothalmus tissues not included in the Allen Mouse Brain Common Coordinate Framework version 3 (CCFv3)	2	73	January 29, 2025
Advice of Lipofectamine STEM protocol hiPSC Culture Discussion	1	270	December 4, 2024
Single cell cluster naming General	1	99	November 27, 2024
Demethylating haldol affected genes Education	4	75	November 12, 2024
Image viewer error classic segmentation jupyter notebook Software & Code segmenter	4	439	August 15, 2024
How to keep cardiac differentiated cells from coming apart lifting from well hiPSC Culture Discussion	1	64	July 30, 2024
Evaluating morphology of hiPS colonies	1	160	July 25, 2024
Drug perturbation single cell segmentation segmentation	1	71	July 2, 2024
How to access cell lines Gene-editing Discussion	1	90	June 20, 2024
Is there any timelapse microscopy data for fluorescently tagged hiPSCs available for download? Download	1	114	May 16, 2024
Facing Problems while working with AICSImage package for CZI files aiscimageio	4	241	April 11, 2024