Allen Cell Discussion Forum

Topic	Replies	Views	Activity
iPSC line AICS-0090-391 adipogenic differentiation hiPSC Culture Discussion	0	9	November 19, 2025
Brain and body donation to Allen Institute to support specified research segmentation	1	19	November 13, 2025
WTC-11 Genome Sequence in the UCSC Browser	3	20	October 8, 2025
Thaw, expand and maintain MONO-ALLELIC mEGFP-TAGGED SOX2 cells in E8+ flex medium (Invitrogen) on Vitronectin-N coated wells hiPSC Culture Discussion getting-started	1	28	July 30, 2025
Recreating gif style figure on Spherical Harmonics read me	1	29	June 26, 2025
Tips for reducing non-specific antibody binding in WTC-11 cells on coated glass hiPSC Culture Discussion	0	35	April 23, 2025
Culturing hiPSC and Other Cell Lines in Same Lab Space hiPSC Culture Discussion	1	56	April 2, 2025
AICS-0126-068:WTC-mEGFP-CDH5 and AICS-0126-041:WTC-mEGFP-CDH5 hiPSC Culture Discussion	1	43	March 20, 2025
(AICS-0074-026) MONO-ALLELIC mEGFP-TAGGED SOX hiPSCs Thawing/freezing question	2	45	March 20, 2025
Cvapipe_analysis, cannot run test case (shape analysis on 300 cells) Software & Code bug-report	1	43	March 19, 2025
Protocol for Parental Cell Line (GM25256) hiPSC Culture Discussion	1	44	February 17, 2025
AICS-0037-172-MONO-ALLELIC mEGFP-TAGGED TNNI iPSCs RFP Signal hiPSC Culture Discussion	1	36	February 4, 2025
Hypothalmus tissues not included in the Allen Mouse Brain Common Coordinate Framework version 3 (CCFv3)	2	39	January 29, 2025
Advice of Lipofectamine STEM protocol hiPSC Culture Discussion	1	107	December 4, 2024
Single cell cluster naming General	1	44	November 27, 2024
Demethylating haldol affected genes Education	4	37	November 12, 2024
Image viewer error classic segmentation jupyter notebook Software & Code segmenter	4	360	August 15, 2024
How to keep cardiac differentiated cells from coming apart lifting from well hiPSC Culture Discussion	1	36	July 30, 2024
Evaluating morphology of hiPS colonies	1	104	July 25, 2024
Drug perturbation single cell segmentation segmentation	1	41	July 2, 2024
How to access cell lines Gene-editing Discussion	1	63	June 20, 2024
Is there any timelapse microscopy data for fluorescently tagged hiPSCs available for download? Download	1	89	May 16, 2024
Facing Problems while working with AICSImage package for CZI files aiscimageio	4	192	April 11, 2024
Found way around not well formed error and itkwidgets error, Classical Segmenter segmentation , getting-started , segmenter	0	133	March 8, 2024
Why python, not imageJ? Miscellaneous	3	1288	March 1, 2024
AICSImage not Defined, Classical Segmenter Software & Code segmentation , getting-started , segmenter	2	149	February 27, 2024
Aicssegmentation not found in Jupyter, Classic Segmentation getting-started , segmenter	3	163	February 24, 2024
Cardiomyocyte Differentiation Issue hiPSC Culture Discussion cardiac	12	1933	February 22, 2024
Commercially available, Genetically Encoded Calcium Indicator stable iPSC-cell line?	1	139	February 9, 2024
Segmenter training data and trained models Software & Code segmenter	2	156	January 10, 2024