When should I use ROCK inhibitor?


A common question we get is about when should media contain ROCK inhibitor (molecule Y-27632)?

As a rule of thumb, cells should be fed media containing ROCK inhibitor 24 hours after thawing, and for 24 hours after each single cell passaging event.

ROCK inhibitor can also be used for a few hours before a “traumatic” cell event like FACS enrichment or transfection to boost viability.