Growing our hiPSC lines on glass for imaging is a little bit different than growing them on plastic. We recommend phenol-red free Matrigel, and only coating plates at room temperature for 1 hour prior to seeding cells. See our protocol SOP: Cell plating for imaging on our website, https://www.allencell.org/methods-for-cells-in-the-lab.html#sops for more details!
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