Growing our hiPSC lines on glass for imaging is a little bit different than growing them on plastic. We recommend phenol-red free Matrigel, and only coating plates at room temperature for 1 hour prior to seeding cells. See our protocol SOP: Cell plating for imaging on our website, https://www.allencell.org/methods-for-cells-in-the-lab.html#sops for more details!
Related topics
| Topic | Replies | Views | Activity | |
|---|---|---|---|---|
| Matrigel dilutions | 0 | 1164 | September 26, 2018 | |
| Thaw, expand and maintain MONO-ALLELIC mEGFP-TAGGED SOX2 cells in E8+ flex medium (Invitrogen) on Vitronectin-N coated wells | 1 | 23 | July 30, 2025 | |
| Reason for Matrigel Concentration Recommended | 1 | 308 | August 16, 2023 | |
| Dilution of Matrigel : which media? | 2 | 363 | January 19, 2023 | |
| Protocol for Parental Cell Line (GM25256) | 1 | 39 | February 17, 2025 |
