Growing our hiPSC lines on glass for imaging is a little bit different than growing them on plastic. We recommend phenol-red free Matrigel, and only coating plates at room temperature for 1 hour prior to seeding cells. See our protocol SOP: Cell plating for imaging on our website, https://www.allencell.org/methods-for-cells-in-the-lab.html#sops for more details!
Related topics
Topic | Replies | Views | Activity | |
---|---|---|---|---|
Matrigel dilutions | 0 | 1146 | September 26, 2018 | |
Thaw, expand and maintain MONO-ALLELIC mEGFP-TAGGED SOX2 cells in E8+ flex medium (Invitrogen) on Vitronectin-N coated wells | 1 | 15 | July 30, 2025 | |
Reason for Matrigel Concentration Recommended | 1 | 282 | August 16, 2023 | |
Dilution of Matrigel : which media? | 2 | 350 | January 19, 2023 | |
Protocol for Parental Cell Line (GM25256) | 1 | 29 | February 17, 2025 |