We take a conservative approach to diluting our Matrigel because of morphology differences we see when our hiPSC are plated on glass. We observe the best morphology on glass with a 1:30 dilution of Matrigel, and our cells are also healthy on this dilution of Matrigel on plastic, so… that’s what we recommend!
Related Topics
| Topic | Replies | Views | Activity | |
|---|---|---|---|---|
| Reason for Matrigel Concentration Recommended | 1 | 225 | August 16, 2023 | |
| Culturing cells for imaging on glass | 1 | 1084 | July 7, 2020 | |
| Conflicting Matrigel instructions | 1 | 307 | November 10, 2022 | |
| Dilution of Matrigel : which media? | 2 | 311 | January 19, 2023 | |
| Matrigel vs geltrex | 3 | 838 | November 10, 2022 |