Silent gene-editing of transcriptionally silent genes in hiPSCs

Gene-editing Discussion
1

In order to tag genes that are expressed upon differentiation, we have created a silent gene-editing approach in human iPSCs using CRISPR/Cas9, and tagged specific cardiac genes. See the full story at https://www.biorxiv.org/content/early/2018/06/09/342881

2

Hello, a really cool technique. I wish to do something similar and was wondering if the donor plasmid with homology arms has been made available to the public? If not, I was wondering if it is possible to get some so we can customize it for our gene of interest.

Thanks

3

Thank you for reaching out, @santhoshks.
We deposit all of our donor plasmids containing gene-specific homology arms, the fluorescent protein, and linker sequence (and selection cassette for silent genes), to addgene:


In addition, the crRNA sequences used the create each cell line are listed in the “Editing Design” tab for each cell line in our Cell Catalog: