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This forum is a place for learning, helping, and sharing experiences of using our publicly-available cell lines, plasmids, genomic data, software, and analytic tools.
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Human iPSC gene-editing techniques, tips, & issues
Human iPSC culture, differentiation techniques, tips, & issues
Label-free, cell segmentation, integrated cell modeling, and machine learning techniques, tips, & issues
Cell biology educational resources, use-cases, & tips
Community Ground Rules & Privacy Statement
Full website of the Allen Institute for Cell Science including pages for Gene-editing Methods and Videos & Tutorials
| Topic | Replies | Views | Activity | |
|---|---|---|---|---|
| Matrigel dilutions | 0 | 1150 | September 26, 2018 | |
| Thaw, expand and maintain MONO-ALLELIC mEGFP-TAGGED SOX2 cells in E8+ flex medium (Invitrogen) on Vitronectin-N coated wells | 1 | 19 | July 30, 2025 | |
| Reason for Matrigel Concentration Recommended | 1 | 292 | August 16, 2023 | |
| Dilution of Matrigel : which media? | 2 | 353 | January 19, 2023 | |
| Protocol for Parental Cell Line (GM25256) | 1 | 32 | February 17, 2025 |
