Hello Adriana!
I’ve included an answer to this question in another thread of yours, but for folks just joining this one, I’ll answer here as well. While I have not tried this personally, my teammate Angel is very familiar with clump passaging and said the following:
While passaging in clumps, you do NOT need ROCK inhibitor. At a freeze or a thaw step, you can use it for added survival, but not at the regular passage steps. One thing to add: if you’re using dispase or collagenase to dissociate, you should add in an extra wash with PBS or media before plating cells.
I hope this helps!
See related thread: Single cell vs colony passaging