Have you guys began hiPSC differentiation in to cardiomyocytes?
Yes! Our method at the Allen Institute for Cell Science was adapted from the small molecule protocol described in Lian et al., Nature Protocols, 2013, and has been optimized for the differentiation of cardiomyocytes in a 6-well format using the Allen Cell Collection, derived from the hiPSC WTC parental line released by the Conklin Laboratory at the J. David Gladstone Institute.
Check out our SOP: Cardiomyocyte differentiation methods v1.0.pdf for more details!
From the Allen Cell Collection, MYL7-mEGFP cell line, Day 12 after differentiation
You can also find our methods video where we set up hiPSCs for cardiac differentiation here:
8 posts were split to a new topic: Cardiomyocyte Differentiation Issue