Hi Haseeb,
Thank you for the protocol! I’ve had some success differentiating commercial iPSC lines using your protocol (>97% cTNT+). I’m currently working on a patient line and I’ve identified a seeding density at which they would produce beating clusters but the beatings are localized on the periphery. I suspect I would only get less than 50% cTNT+ cells.
What do you suggest I should do next? Should I increase the Chiron concentration or change the seeding density?
Thank you! I really appreciate all your help with this.