Hi, sorry for this maybe very basic question. I’m very new to image processing.
I downloaded the label free tom20 images from the website: https://www.allencell.org/data-downloading.html#sectionLabelFreeTrainingData
When I used aicsimageio package to read the czi files, I found that each image has 7 channels. [‘CMDRP_1’, ‘CMDRP’, ‘EGFP_1’, ‘EGFP’, ‘H3342_1’, ‘H3342’, ‘Bright_100X’]
and the metadata for a specific image is:
<Element ImageDocument at 0x12de47eb0>
My question is I can’t find specific information about what each channel means.
I know that EGFP stains for the mitochondria, H3342 for DNA and CMDRP for membrane. But I don’t know what’s the difference with EGFP and EGFP_1.
After looking into actual pixel values of each channel, my assumption is that EGFP_1 is the channel for the stain, and EGFP is the binary segmentation channel with cell numbers.
Is there anyway I can find documentation or someone to confirm this for me?
Thank you so much!!