I have tried using the spherical harmonics method for retinal pigment epithelial tissue cells . The cells are hexagonal and spherical harmonics seem to not capture this shape easily due to the existence of sharp edges. Is there a modification to the method that can capture this information?
Also, 3-5% of our cells may have multiple nuclei. How do you deal with the parametrization since the method is based on single nucleus?
I would greatly appreciate if there are any suggestions or leads that you may share!