I found the workflows that you share very nicely on your website.
I would like to do segmentation on cells (3d and 2d) mainly to segment the nucleus (DAPI), FISH signal (3d), 3d dots and membranes.
Looking at the # Lookup table of classic image segmentation workflows for 28 intracellular structure localization patterns#
I can easily find which one corresponds to the membrane and the 3d spots BUT I cant find which one is relevant for nucleus segmentation using DAPI (2d / 3d).
My workflow will be :
segmentation of the nucleus (2d / 3d)
Segmentation of the dots 2d / 3d.
hence the first step is to segment the nucleus,
Could you provide me some info or point me into the right direction ?
Thanks a lot,