Allen Cell Discussion Forum

Pictures of Healthy vs Unhealthy hiPSC colonies

How should healthy iPSC colonies look? What characteristics define a bad colony?

Example of a crater formation in hiPSC colonies, not healthy!

Healthier hiPSC colonies below, notice the smooth edges:

hiPSC colonies undergoing differentiation, look for triangular or spiky cells at edges:
differentiation_4x3-01

Underconfluence of hiPSC colonies:
underconfluent3-01

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Is there somewhere on the AICS website where I can find a collection of healthy and unhealthy colony images suitable for benchmarking a machine-learning based classifier?

Hi @gregj, We do not have a set at this moment available on our website. However, maybe one of our scientists may be able to provide images. About how many images of healthy and unhealthy would you need for machine learning?

Thanks!