How do you test for pluripotency of the hiPSCs after editing?


#1

After you edit the cells, how do you test for pluripotency of the hiPSCs?


#2

We look for expression of pluripotency markers like Oct3/4, Sox2, and Nanog by flow cytometry. We also differentiate iPSCs into the three germ layers using a commercial kit and look for markers of ectoderm, endoderm and mesoderm by droplet digital PCR or flow cytometry. A more detailed look at our methods can be found here: https://www.biorxiv.org/content/early/2018/06/09/342881