I am trying to segment Lamin B1 channel. I followed the AICS laminB1 notebook. The cross sections seem decent quality but I am unable to get the top and bottom layers like shown in the example in this page. What can I do to get clean segmentation of top and bottom parts of laminB1. I also tried 3d filament instead of 2d filament, but the suggested 2d filament appears to be better quality.