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(1) make sure your file realy exists
(2) your file path does not seem to be correct full path, should it be something like C://Users/…
(3) you can also try a relative path, namely, copying your tiff file to the same directory as where you start your jupyter. then, use filename only to load the image
In a normal string, try doubling the backslashes in your paths. For example, ’\Users\Desktop\AICSTest.tif’ becomes ‘\\Users\\Desktop\\AICSTest.tif’. Python interprets a single backslash as an “escape” character and is trying to interpret “\U” as a special thing. Your “r” string would have worked but I believe was missing the C: drive designator, as Jianxu mentioned. You should then be able to load files from your desktop.