# \#getting-started

**URL:** https://forum.allencell.org/tag/getting-started/8.md

[Latest](https://forum.allencell.org/latest.md) · [Categories](https://forum.allencell.org/categories.md) · [Tags](https://forum.allencell.org/tags.md)

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## [FNet setup issues due to outdated dependencies – any updated version or Docker?](https://forum.allencell.org/t/fnet-setup-issues-due-to-outdated-dependencies-any-updated-version-or-docker/792)

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**Author:** [@ines\_varona](https://forum.allencell.org/u/ines_varona)\
**Replies:** 5\
**Last updated:** [March 25, 2026, 11:31pm UTC](https://forum.allencell.org/t/fnet-setup-issues-due-to-outdated-dependencies-any-updated-version-or-docker/792 "2026-03-25T23:31:18Z")

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Hi everyone, I am currently running some experiments and would like to compare my results with outputs obtained using the FNet architecture. However, while setting up the environment and installing the required depende…

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## [Thaw, expand and maintain MONO-ALLELIC mEGFP-TAGGED SOX2 cells in E8+ flex medium (Invitrogen) on Vitronectin-N coated wells](https://forum.allencell.org/t/thaw-expand-and-maintain-mono-allelic-megfp-tagged-sox2-cells-in-e8-flex-medium-invitrogen-on-vitronectin-n-coated-wells/771)

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**Author:** [@leahpeng](https://forum.allencell.org/u/leahpeng)\
**Replies:** 1\
**Last updated:** [July 30, 2025, 8:09pm UTC](https://forum.allencell.org/t/thaw-expand-and-maintain-mono-allelic-megfp-tagged-sox2-cells-in-e8-flex-medium-invitrogen-on-vitronectin-n-coated-wells/771 "2025-07-30T20:09:44Z")

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Hi, We would like to thaw, expand and maintain MONO-ALLELIC mEGFP-TAGGED SOX2 cells in E8+ flex medium (Invitrogen) on Vitronectin-N coated wells, which is different method comparing to the protocol provided by the Cori…

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## [Found way around not well formed error and itkwidgets error, Classical Segmenter](https://forum.allencell.org/t/found-way-around-not-well-formed-error-and-itkwidgets-error-classical-segmenter/685)

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**Author:** [@jcvance](https://forum.allencell.org/u/jcvance)\
**Replies:** 0\
**Last updated:** [March 8, 2024, 4:18pm UTC](https://forum.allencell.org/t/found-way-around-not-well-formed-error-and-itkwidgets-error-classical-segmenter/685 "2024-03-08T16:18:46Z")

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Hi all, I was having an issue running demo\_TNNI1 on jupyter notebook for the classical segmenter. I am running it on Windows. When reading the TNNI1 data file, I got this note: “Failed to parse XML for the provided file…

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## [AICSImage not Defined, Classical Segmenter](https://forum.allencell.org/t/aicsimage-not-defined-classical-segmenter/683)

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**Author:** [@jcvance](https://forum.allencell.org/u/jcvance)\
**Replies:** 2\
**Last updated:** [February 27, 2024, 9:54pm UTC](https://forum.allencell.org/t/aicsimage-not-defined-classical-segmenter/683 "2024-02-27T21:54:07Z")

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Hi all, I am having difficulty running playground\_npm1. After loading the image, I get this error: NameError Traceback (most recent call last) ~\\AppData\\Local\\Temp\\ipykernel\_28628\\1638…

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## [Aicssegmentation not found in Jupyter, Classic Segmentation](https://forum.allencell.org/t/aicssegmentation-not-found-in-jupyter-classic-segmentation/681)

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**Author:** [@jcvance](https://forum.allencell.org/u/jcvance)\
**Replies:** 3\
**Last updated:** [February 24, 2024, 12:11am UTC](https://forum.allencell.org/t/aicssegmentation-not-found-in-jupyter-classic-segmentation/681 "2024-02-24T00:11:31Z")

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Hi, I am new to python and jupyter. I have been getting this error in demo\_TNNI1 on jupyter notebook: ModuleNotFoundError Traceback (most recent call last) ~\\AppData\\Local\\Temp\\ipykernel\_8960\\3994…

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## [Downloading Cell Feature Data](https://forum.allencell.org/t/downloading-cell-feature-data/656)

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**Author:** [@mtu2](https://forum.allencell.org/u/mtu2)\
**Replies:** 10\
**Last updated:** [September 8, 2023, 7:19pm UTC](https://forum.allencell.org/t/downloading-cell-feature-data/656 "2023-09-08T19:19:46Z")

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Hi I’m new here and I am looking for cell morphology data such that I have a csv where each row is a cell and each column is a shape property(eg. area, perimeter). I don’t know exactly how to search for what I need on q…

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## ["setup.py" not found error](https://forum.allencell.org/t/setup-py-not-found-error/354)

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**Author:** [@AidanF](https://forum.allencell.org/u/AidanF)\
**Replies:** 1\
**Last updated:** [September 9, 2021, 5:18pm UTC](https://forum.allencell.org/t/setup-py-not-found-error/354 "2021-09-09T17:18:26Z")

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Hi All, Getting the segmenter up and running on a new computer. No issues previously but running into this issue now when I issue the following command: "pip install -e .\[all\] ERROR: File “setup.py” not found. Director…

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## [Cvapipeline\_analysis - Can I download a smaller dataset from Quilt to test the integrated modeling and analysis pipeline?](https://forum.allencell.org/t/cvapipeline-analysis-can-i-download-a-smaller-dataset-from-quilt-to-test-the-integrated-modeling-and-analysis-pipeline/278)

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**Author:** [@MaryMirvis](https://forum.allencell.org/u/MaryMirvis)\
**Replies:** 5\
**Last updated:** [February 18, 2021, 12:05am UTC](https://forum.allencell.org/t/cvapipeline-analysis-can-i-download-a-smaller-dataset-from-quilt-to-test-the-integrated-modeling-and-analysis-pipeline/278 "2021-02-18T00:05:25Z")

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Hello, I would like to follow the steps in cvapipeline\_analysis to better understand the integrated cell modeling and analysis workflow. However, cvapipe\_analysis loaddata run downloads 7.3 TB of data. I don’t have tha…

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## [Getting started with forum basics](https://forum.allencell.org/t/getting-started-with-forum-basics/224)

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**Author:** [@cells](https://forum.allencell.org/u/cells)\
**Replies:** 0\
**Last updated:** [July 9, 2020, 9:35pm UTC](https://forum.allencell.org/t/getting-started-with-forum-basics/224 "2020-07-09T21:35:41Z")

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Getting Started As a first-time user, you may notice guides/alerts that popup as you begin to explore the forum site. These notes will automatically close after a short time, or you may close them manually. You can brows…

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## [Quick Markdown guide for editing text in posts](https://forum.allencell.org/t/quick-markdown-guide-for-editing-text-in-posts/229)

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**Author:** [@cells](https://forum.allencell.org/u/cells)\
**Replies:** 0\
**Last updated:** [July 20, 2020, 4:26am UTC](https://forum.allencell.org/t/quick-markdown-guide-for-editing-text-in-posts/229 "2020-07-20T04:26:52Z")

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Some common markups: \*italic\* italic \*\*bold \*\* bold #Heading 1 Heading 1 ##Heading 2 Heading 2 \[Link\](link URL) Link \[Image|Size\](Image URL) \>block quote blockquote \* List - List List Horizon…

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## [Forum Navigation](https://forum.allencell.org/t/forum-navigation/225)

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**Author:** [@cells](https://forum.allencell.org/u/cells)\
**Replies:** 0\
**Last updated:** [July 9, 2020, 10:05pm UTC](https://forum.allencell.org/t/forum-navigation/225 "2020-07-09T22:05:32Z")

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Home = Landing page = Latest update page You can always click on the Allen Cell Discussion Forum text link to get back to the Forum homepage. The “hamburger” menu An icon made of 3 horizontal lines is called a “ha…

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## [Forum registration](https://forum.allencell.org/t/forum-registration/223)

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**Author:** [@cells](https://forum.allencell.org/u/cells)\
**Replies:** 0\
**Last updated:** [July 7, 2020, 11:12pm UTC](https://forum.allencell.org/t/forum-registration/223 "2020-07-07T23:12:56Z")

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Begin by clicking the teal \[Sign Up\] button in the right corner of the title banner seen on every forum webpage. The popup window gives you the option to sign up by creating a new account using your email. A…

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## [The training images for the label free prediction](https://forum.allencell.org/t/the-training-images-for-the-label-free-prediction/237)

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**Author:** [@Hanxix](https://forum.allencell.org/u/Hanxix)\
**Replies:** 1\
**Last updated:** [August 13, 2020, 9:22pm UTC](https://forum.allencell.org/t/the-training-images-for-the-label-free-prediction/237 "2020-08-13T21:22:56Z")

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Hi, sorry for this maybe very basic question. I’m very new to image processing. I downloaded the label free tom20 images from the website: https://www.allencell.org/data-downloading.html#sectionLabelFreeTrainingData Wh…

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## [Downloading LabelFree Image From Quilt](https://forum.allencell.org/t/downloading-labelfree-image-from-quilt/234)

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**Author:** [@Hanxix](https://forum.allencell.org/u/Hanxix)\
**Replies:** 4\
**Last updated:** [August 10, 2020, 2:10pm UTC](https://forum.allencell.org/t/downloading-labelfree-image-from-quilt/234 "2020-08-10T14:10:17Z")

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Hi, I would like to download the images from this page: https://open.quiltdata.com/b/allencell/packages/aics/label-free-imaging-collection I was able to browse the data manifest. If I would like to download part of th…

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## [Error Loading Images in Look Up Table](https://forum.allencell.org/t/error-loading-images-in-look-up-table/231)

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**Author:** [@AidanF](https://forum.allencell.org/u/AidanF)\
**Replies:** 6\
**Last updated:** [July 22, 2020, 11:32pm UTC](https://forum.allencell.org/t/error-loading-images-in-look-up-table/231 "2020-07-22T23:32:19Z")

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Hi all, I’ve run into loading images in the look up table demo. Here’s the code: Loading the data FILE\_NAME = ‘\\Users\\Desktop\\AICSTest.tif’ reader = AICSImage(FILE\_NAME) IMG = reader.data.astype(np.float32) print(I…
