# When should I use ROCK inhibitor? **URL:** \ **Category:** hiPSC Culture Discussion\ **Created:** [September 26, 2018, 6:33pm UTC](https://forum.allencell.org/t/when-should-i-use-rock-inhibitor/30 "2018-09-26T18:33:50Z")\ **Posts on this page:** 1\ **Showing post:** 3
**Author:** ![maggiefuqua](https://avatars.discourse-cdn.com/v4/letter/m/fbc32d/32.png) [@maggiefuqua](https://forum.allencell.org/u/maggiefuqua)\ **Post date:** [December 7, 2019, 11:55pm UTC](https://forum.allencell.org/t/when-should-i-use-rock-inhibitor/30/3 "2019-12-07T23:55:33Z")
Hello Adriana! I’ve included an answer to this question in another thread of yours, but for folks just joining this one, I’ll answer here as well. While I have not tried this personally, my teammate Angel is very familiar with clump passaging and said the following: While passaging in clumps, you do NOT need ROCK inhibitor. At a freeze or a thaw step, you can use it for added survival, but not at the regular passage steps. One thing to add: if you’re using dispase or collagenase to dissociate, you should add in an extra wash with PBS or media before plating cells. I hope this helps! _See related thread: [Single cell vs colony passaging](https://forum.allencell.org/t/single-cell-vs-colony-passaging/150)_ --- _[View the full topic](https://forum.allencell.org/t/when-should-i-use-rock-inhibitor/30)._