# Weekend free protocols for iPS maintenance

**URL:** <https://forum.allencell.org/t/weekend-free-protocols-for-ips-maintenance/586>\
**Category:** hiPSC Culture Discussion\
**Created:** [January 12, 2023, 4:08pm UTC](https://forum.allencell.org/t/weekend-free-protocols-for-ips-maintenance/586 "2023-01-12T16:08:10Z")\
**Posts on this page:** 4\
**Page:** 1

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**Author:** ![hperera](https://avatars.discourse-cdn.com/v4/letter/h/ea666f/32.png) [@hperera](https://forum.allencell.org/u/hperera)\
**Post date:** [January 12, 2023, 4:08pm UTC](https://forum.allencell.org/t/weekend-free-protocols-for-ips-maintenance/586/1 "2023-01-12T16:08:10Z")

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Can I passage cells on a Thursday or a Friday and do a double feed on a Saturday so that I can skip a feed on a Sunday?

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**Author:** ![en2b](https://avatars.discourse-cdn.com/v4/letter/e/da6949/32.png) [@en2b](https://forum.allencell.org/u/en2b)\
**Post date:** [January 24, 2023, 9:36pm UTC](https://forum.allencell.org/t/weekend-free-protocols-for-ips-maintenance/586/2 "2023-01-24T21:36:34Z")

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Hi hperera! I routinely passage my cells on Thursday at a density where I can double feed on Friday and passage again on Monday (no feeding required on Saturday OR Sunday- hooray!).

For cell lines that do **not** require ROCK inhibitor when passaging (such as the parental WTC11s), I have also had success passaging on Friday directly into 6-well plates containing 4mL of medium per well and incubating until Monday.

Note: I use mTeSR Plus medium

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**Author:** ![hperera](https://avatars.discourse-cdn.com/v4/letter/h/ea666f/32.png) [@hperera](https://forum.allencell.org/u/hperera)\
**Post date:** [March 18, 2023, 11:16am UTC](https://forum.allencell.org/t/weekend-free-protocols-for-ips-maintenance/586/3 "2023-03-18T11:16:11Z")

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Hi en2b!

Thank you for the information. What is the seeding density that you use for 6well plates? when passaging on Friday?

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**Author:** ![Janani](https://avatars.discourse-cdn.com/v4/letter/j/f14d63/32.png) [@Janani](https://forum.allencell.org/u/Janani)\
**Post date:** [April 20, 2023, 5:37pm UTC](https://forum.allencell.org/t/weekend-free-protocols-for-ips-maintenance/586/4 "2023-04-20T17:37:56Z")

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Hi @hperera,

We typically seed 150K cells for 3 days, 75K for 4 days in 6 well plates. We find that growth is more dense at perimeter of the well and uneven at the center.

Thanks,  
Janani