# Silent gene-editing of transcriptionally silent genes in hiPSCs

**URL:** <https://forum.allencell.org/t/silent-gene-editing-of-transcriptionally-silent-genes-in-hipscs/27>\
**Category:** Gene-editing Discussion\
**Created:** [September 25, 2018, 8:38pm UTC](https://forum.allencell.org/t/silent-gene-editing-of-transcriptionally-silent-genes-in-hipscs/27 "2018-09-25T20:38:11Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![cells](https://avatars.discourse-cdn.com/v4/letter/c/ecc23a/32.png) [@cells](https://forum.allencell.org/u/cells)\
**Post date:** [September 25, 2018, 8:38pm UTC](https://forum.allencell.org/t/silent-gene-editing-of-transcriptionally-silent-genes-in-hipscs/27/1 "2018-09-25T20:38:11Z")

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In order to tag genes that are expressed upon differentiation, we have created a silent gene-editing approach in human iPSCs using CRISPR/Cas9, and tagged specific cardiac genes. See the full story at [https://www.biorxiv.org/content/early/2018/06/09/342881](https://www.biorxiv.org/content/early/2018/06/09/342881)

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**Author:** ![santhoshks](https://avatars.discourse-cdn.com/v4/letter/s/ecd19e/32.png) [@santhoshks](https://forum.allencell.org/u/santhoshks)\
**Post date:** [December 3, 2020, 5:01pm UTC](https://forum.allencell.org/t/silent-gene-editing-of-transcriptionally-silent-genes-in-hipscs/27/2 "2020-12-03T17:01:59Z")

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Hello, a really cool technique. I wish to do something similar and was wondering if the donor plasmid with homology arms has been made available to the public? If not, I was wondering if it is possible to get some so we can customize it for our gene of interest.

Thanks

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**Author:** ![irinam](https://avatars.discourse-cdn.com/v4/letter/i/c37758/32.png) [@irinam](https://forum.allencell.org/u/irinam)\
**Post date:** [December 4, 2020, 12:53am UTC](https://forum.allencell.org/t/silent-gene-editing-of-transcriptionally-silent-genes-in-hipscs/27/3 "2020-12-04T00:53:10Z")

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Thank you for reaching out, @santhoshks.  
We deposit all of our donor plasmids containing gene-specific homology arms, the fluorescent protein, and linker sequence (and selection cassette for silent genes), to addgene:

> **[Addgene: The Allen Institute for Cell Science Lab Plasmids](https://www.addgene.org/The_Allen_Institute_for_Cell_Science/)**

  
In addition, the crRNA sequences used the create each cell line are listed in the “Editing Design” tab for each cell line in our Cell Catalog:  

> **[Cell Catalog](https://www.allencell.org/cell-catalog.html)**
>
> We have two new fluorescently tagged hiPSC lines now available in the Allen Cell Collection. The first is a cardiac and skeletal muscle-specific line in which dystrophin is tagged with mEGFP. The...