# sgRNA transfection

**URL:** <https://forum.allencell.org/t/sgrna-transfection/603>\
**Category:** Gene-editing Discussion\
**Created:** [January 23, 2023, 8:01pm UTC](https://forum.allencell.org/t/sgrna-transfection/603 "2023-01-23T20:01:20Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![en2b](https://avatars.discourse-cdn.com/v4/letter/e/da6949/32.png) [@en2b](https://forum.allencell.org/u/en2b)\
**Post date:** [January 23, 2023, 8:01pm UTC](https://forum.allencell.org/t/sgrna-transfection/603/1 "2023-01-23T20:01:20Z")

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I am planning to transfect the WTC11-dCas9-KRAB cell line with sgRNA for gene knockdown. Do you have a recommended amount of sgRNA to transfect when the cells are already expressing dCas9?