# RNA sequences from layer 23 pyramidal neuron

**URL:** <https://forum.allencell.org/t/rna-sequences-from-layer-23-pyramidal-neuron/604>\
**Category:** General\
**Tags:** sequencing\
**Created:** [January 24, 2023, 6:51pm UTC](https://forum.allencell.org/t/rna-sequences-from-layer-23-pyramidal-neuron/604 "2023-01-24T18:51:30Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![tom](https://avatars.discourse-cdn.com/v4/letter/t/3d9bf3/32.png) [@tom](https://forum.allencell.org/u/tom)\
**Post date:** [January 24, 2023, 6:51pm UTC](https://forum.allencell.org/t/rna-sequences-from-layer-23-pyramidal-neuron/604/1 "2023-01-24T18:51:30Z")

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If such a thing exists here or elsewhere, I was wondering if anyone can point me to a link to a web page or file of (gene name annotated if possible) expressed RNA sequences from layer 23 pyramidal neuron(s) in mice?

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**Author:** ![tom](https://avatars.discourse-cdn.com/v4/letter/t/3d9bf3/32.png) [@tom](https://forum.allencell.org/u/tom)\
**Post date:** [June 24, 2023, 11:08pm UTC](https://forum.allencell.org/t/rna-sequences-from-layer-23-pyramidal-neuron/604/2 "2023-06-24T23:08:03Z")

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With a literature search I found a paper  
Liu J, Wang M, Sun L, Pan NC, Zhang C, Zhang J, Zuo Z, He S, Wu Q, Wang X. Integrative analysis of in vivo recording with single-cell RNA-seq data reveals molecular properties of light-sensitive neurons in mouse V1. Protein Cell. 2020 Jun;11(6):417-432. doi: 10.1007/s13238-020-00720-y. Epub 2020 Apr 29. PMID: 32350740; PMCID: PMC7251024.  
whose data availability statement cited the Gene Expression Omnibus database

> **[GEO Accession viewer](https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE115997)**
>
> NCBI's Gene Expression Omnibus (GEO) is a public archive and resource for gene expression data.

Some local to my institution bioinformatics experts showed me that this “count\_text” file (link from the bottom of the above page) contained the expression data I was looking for: [https://www.ncbi.nlm.nih.gov/geo/download/?acc=GSE115997&format=file&file=GSE115997\_count.txt.gz](https://www.ncbi.nlm.nih.gov/geo/download/?acc=GSE115997&format=file&file=GSE115997%5Fcount%2Etxt%2Egz)