# Matrigel dilutions

**URL:** <https://forum.allencell.org/t/matrigel-dilutions/31>\
**Category:** hiPSC Culture Discussion\
**Tags:** matrigel\
**Created:** [September 26, 2018, 6:41pm UTC](https://forum.allencell.org/t/matrigel-dilutions/31 "2018-09-26T18:41:12Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![amandah](https://avatars.discourse-cdn.com/v4/letter/a/e47774/32.png) [@amandah](https://forum.allencell.org/u/amandah)\
**Post date:** [September 26, 2018, 6:41pm UTC](https://forum.allencell.org/t/matrigel-dilutions/31/1 "2018-09-26T18:41:12Z")

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We take a conservative approach to diluting our Matrigel because of morphology differences we see when our hiPSC are plated on glass. We observe the best morphology on glass with a 1:30 dilution of Matrigel, and our cells are also healthy on this dilution of Matrigel on plastic, so… that’s what we recommend!
