# Lamin B1 Segmentation

**URL:** <https://forum.allencell.org/t/lamin-b1-segmentation/247>\
**Category:** Segmenter\
**Tags:** segmentation\
**Created:** [August 24, 2020, 12:18am UTC](https://forum.allencell.org/t/lamin-b1-segmentation/247 "2020-08-24T00:18:58Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![pushkar](https://yyz1.discourse-cdn.com/flex027/user_avatar/forum.allencell.org/pushkar/32/91_2.png) [@pushkar](https://forum.allencell.org/u/pushkar)\
**Post date:** [August 24, 2020, 12:18am UTC](https://forum.allencell.org/t/lamin-b1-segmentation/247/1 "2020-08-24T00:18:58Z")

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Hi,  
I am trying to segment Lamin B1 channel. I followed the AICS laminB1 notebook. The cross sections seem decent quality but I am unable to get the top and bottom layers like shown in the example in [this page](https://www.allencell.org/segmenter.html). What can I do to get clean segmentation of top and bottom parts of laminB1. I also tried 3d filament instead of 2d filament, but the suggested 2d filament appears to be better quality.

Thanks,  
Pushkar

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**Author:** ![jianxu\_chen](https://avatars.discourse-cdn.com/v4/letter/j/85f322/32.png) [@jianxu\_chen](https://forum.allencell.org/u/jianxu_chen)\
**Post date:** [August 30, 2020, 2:31am UTC](https://forum.allencell.org/t/lamin-b1-segmentation/247/2 "2020-08-30T02:31:41Z")

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there are lots of factors that can affect the top and bottom segmentation quality.

first of all, what is the z resolution of your images? Since lamin shells could be very thin, insufficient resolution along z can lead to bad segmentation near top and bottom. If you do a side view of your image in imageJ, you can have some idea about how it looks.

If you need further help, you may post a few examples here, like XY view and YZ view of the image.

Hope it helps.  
Jianxu