# iPS cardiac differentiation with lactate

**URL:** <https://forum.allencell.org/t/ips-cardiac-differentiation-with-lactate/664>\
**Category:** hiPSC Culture Discussion\
**Created:** [November 1, 2023, 6:29pm UTC](https://forum.allencell.org/t/ips-cardiac-differentiation-with-lactate/664 "2023-11-01T18:29:35Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![hartw006](https://avatars.discourse-cdn.com/v4/letter/h/ed655f/32.png) [@hartw006](https://forum.allencell.org/u/hartw006)\
**Post date:** [November 1, 2023, 6:29pm UTC](https://forum.allencell.org/t/ips-cardiac-differentiation-with-lactate/664/1 "2023-11-01T18:29:35Z")

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Hi,  
Can you suggest a protocol for lactate purification after wnt-modulated differentiation?  
I have been unable to find a detailed protocol.  
thanks,  
Lynn

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**Author:** ![becky.zaunbrecher](https://avatars.discourse-cdn.com/v4/letter/b/f4b2a3/32.png) [@becky.zaunbrecher](https://forum.allencell.org/u/becky.zaunbrecher)\
**Post date:** [November 1, 2023, 9:26pm UTC](https://forum.allencell.org/t/ips-cardiac-differentiation-with-lactate/664/2 "2023-11-01T21:26:19Z")

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Hi @hartw006 ,

Thanks for your question - we haven’t used lactate selection to purify our hiPSC-derived cardios here at AICS, but here is a paper which describes a method one of my colleagues has used in her previous lab:

[https://www.sciencedirect.com/science/article/pii/S2211124720309062?via%3Dihub](https://www.sciencedirect.com/science/article/pii/S2211124720309062?via%3Dihub)

Particularly, the description under the section " Human iPSC culture and differentiation" in the Methods section has a detailed description of the lactate selection method used.

Thanks & best,  
Becky Zaunbrecher