# How do you test for pluripotency of the hiPSCs after editing?

**URL:** <https://forum.allencell.org/t/how-do-you-test-for-pluripotency-of-the-hipscs-after-editing/40>\
**Category:** Gene-editing Discussion\
**Created:** [September 27, 2018, 4:50pm UTC](https://forum.allencell.org/t/how-do-you-test-for-pluripotency-of-the-hipscs-after-editing/40 "2018-09-27T16:50:40Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![kim.cordes](https://avatars.discourse-cdn.com/v4/letter/k/fbc32d/32.png) [@kim.cordes](https://forum.allencell.org/u/kim.cordes)\
**Post date:** [September 27, 2018, 4:50pm UTC](https://forum.allencell.org/t/how-do-you-test-for-pluripotency-of-the-hipscs-after-editing/40/1 "2018-09-27T16:50:40Z")

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After you edit the cells, how do you test for pluripotency of the hiPSCs?

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**Author:** ![joy](https://avatars.discourse-cdn.com/v4/letter/j/b77776/32.png) [@joy](https://forum.allencell.org/u/joy)\
**Post date:** [September 28, 2018, 7:17pm UTC](https://forum.allencell.org/t/how-do-you-test-for-pluripotency-of-the-hipscs-after-editing/40/2 "2018-09-28T19:17:08Z")

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We look for expression of pluripotency markers like Oct3/4, Sox2, and Nanog by flow cytometry. We also differentiate iPSCs into the three germ layers using a commercial kit and look for markers of ectoderm, endoderm and mesoderm by droplet digital PCR or flow cytometry. A more detailed look at our methods can be found here: [https://www.biorxiv.org/content/early/2018/06/09/342881](https://www.biorxiv.org/content/early/2018/06/09/342881)