# Dilution of Matrigel : which media?

**URL:** <https://forum.allencell.org/t/dilution-of-matrigel-which-media/595>\
**Category:** hiPSC Culture Discussion\
**Created:** [January 18, 2023, 8:01am UTC](https://forum.allencell.org/t/dilution-of-matrigel-which-media/595 "2023-01-18T08:01:02Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![OBEID](https://avatars.discourse-cdn.com/v4/letter/o/8dc957/32.png) [@OBEID](https://forum.allencell.org/u/OBEID)\
**Post date:** [January 18, 2023, 8:01am UTC](https://forum.allencell.org/t/dilution-of-matrigel-which-media/595/1 "2023-01-18T08:01:02Z")

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I have a doubt…  
In the protocol version 1.7, I read that you have to dilute the Matrigel in DMEM/F12 to make the coating. But in paragraph 13 of the Passaging section, I understand that the Matrigel is prepared in mTeSR1 + Ri.  
Can you please clarify this point?  
Thank you in advance and have a nice day  
Patricia

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**Author:** ![jackie\_edmonds](https://avatars.discourse-cdn.com/v4/letter/j/90db22/32.png) [@jackie\_edmonds](https://forum.allencell.org/u/jackie_edmonds)\
**Post date:** [January 19, 2023, 12:37am UTC](https://forum.allencell.org/t/dilution-of-matrigel-which-media/595/2 "2023-01-19T00:37:09Z")

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Hi @OBEID !

You are correct, to make the coating you should dilute Matrigel in DMEM/F12! Paragraph 13 in the passaging section says “Matrigel-coated vessels cannot be re-used, so always seed cells onto fresh Matrigel coated vessels prepared with RT mTeSR1 + Ri.” This means that you should prepare your cell suspension solution in mTeSR1 + Ri and then should seed that cell solution onto a vessel coated with Matrigel (with the Matrigel diluted in DMEM/F12). I hope this helps, please reach out if you have any more questions!

-Jackie

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**Author:** ![OBEID](https://avatars.discourse-cdn.com/v4/letter/o/8dc957/32.png) [@OBEID](https://forum.allencell.org/u/OBEID)\
**Post date:** [January 19, 2023, 5:10am UTC](https://forum.allencell.org/t/dilution-of-matrigel-which-media/595/3 "2023-01-19T05:10:07Z")

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Thanks a lot Jackie  
This confirms what I thought was correct but in order not to compromise my cells I preferred to remove this doubt.  
Good day to you  
Pat