# Cryo-preserving in CryoStor?

**URL:** <https://forum.allencell.org/t/cryo-preserving-in-cryostor/596>\
**Category:** hiPSC Culture Discussion\
**Created:** [January 18, 2023, 9:02am UTC](https://forum.allencell.org/t/cryo-preserving-in-cryostor/596 "2023-01-18T09:02:31Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![OBEID](https://avatars.discourse-cdn.com/v4/letter/o/8dc957/32.png) [@OBEID](https://forum.allencell.org/u/OBEID)\
**Post date:** [January 18, 2023, 9:02am UTC](https://forum.allencell.org/t/cryo-preserving-in-cryostor/596/1 "2023-01-18T09:02:31Z")

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I would like to know if i can cryo-preserve my cell line (AISC-0012) in CryoStorCS10 from StemCell Technologies (#07959).  
Thank you  
Pat

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**Author:** ![j\_smith](https://avatars.discourse-cdn.com/v4/letter/j/c2a13f/32.png) [@j\_smith](https://forum.allencell.org/u/j_smith)\
**Post date:** [January 19, 2023, 5:29pm UTC](https://forum.allencell.org/t/cryo-preserving-in-cryostor/596/2 "2023-01-19T17:29:38Z")

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Pat,

We have not tested long term storage in CryoStorCS10 from Stem Cell Technologies. We have only used CryoStor in our previous method of freezing clones for a short term storage (2 to 3 months at -80C). However, from my understanding when talking to a rep at Stem Cell Technologies at ISSCR last year I was told that you can use CryoStor for long term storage in LN2. I do have plans to test this in the future with our cells but have not done so. I would suggest if you do try to freeze the cells in CryoStor I would also have a back up bank frozen in the standard freeze media consisting of 60% mTeSR1 with 30% KSR, and 10% DMSO. I would suggest you do a side by side recovery to observe morphology and would also suggest checking the cells maintain a normal karyotype. I hope this helps answer your question and feel free to reach out for an further clarification.

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**Author:** ![OBEID](https://avatars.discourse-cdn.com/v4/letter/o/8dc957/32.png) [@OBEID](https://forum.allencell.org/u/OBEID)\
**Post date:** [January 20, 2023, 4:00am UTC](https://forum.allencell.org/t/cryo-preserving-in-cryostor/596/3 "2023-01-20T04:00:25Z")

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> [@OBEID](#):
>
> AISC-0012

Hello  
Thank you for these clarifications. I will do some tests.  
Good day to you.  
Pat