# Conflicting Matrigel instructions

**URL:** <https://forum.allencell.org/t/conflicting-matrigel-instructions/549>\
**Category:** hiPSC Culture Discussion\
**Created:** [November 10, 2022, 3:58pm UTC](https://forum.allencell.org/t/conflicting-matrigel-instructions/549 "2022-11-10T15:58:44Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![en2b](https://avatars.discourse-cdn.com/v4/letter/e/da6949/32.png) [@en2b](https://forum.allencell.org/u/en2b)\
**Post date:** [November 10, 2022, 3:58pm UTC](https://forum.allencell.org/t/conflicting-matrigel-instructions/549/1 "2022-11-10T15:58:45Z")

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I recently received a new vial of cells and the single sheet titled “Allen Cell Collection: Getting Started” states to coat TC plates with Matrigel diluted 1:30. However, the Culture and Freezing Methods for WTC Derived AICS hiPSC Lines documents states that the GFR Matrigel final protein concentration should be 0.337mg/mL. Could you please clarify: are there 2 different Matrigel concentrations used (one for initially thawed cells and one for subsequently passaged cells), or are you working with a lot of GFR Matrigel in which a 1:30 dilution results in a concentration of 0.337mg/mL?

Thank you!

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**Author:** ![Janani](https://avatars.discourse-cdn.com/v4/letter/j/f14d63/32.png) [@Janani](https://forum.allencell.org/u/Janani)\
**Post date:** [November 10, 2022, 7:51pm UTC](https://forum.allencell.org/t/conflicting-matrigel-instructions/549/2 "2022-11-10T19:51:07Z")

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Dear @en2b,

Thank you for reaching out. The matrigel concentration we use is always 0.337mg/mL. The dilution may vary between lots. We make up the same concentration from each lot. Hope this helps. Please reach out if you have more questions.

Janani