# hiPSC Culture Discussion **URL:** https://forum.allencell.org/c/hipsc-culture/10.md?page=1 [Latest](https://forum.allencell.org/latest.md) · [Categories](https://forum.allencell.org/categories.md) · [Tags](https://forum.allencell.org/tags.md) **Page:** 2 --- ## [Culturing cells for imaging on glass](https://forum.allencell.org/t/culturing-cells-for-imaging-on-glass/32)
**Author:** [@amandah](https://forum.allencell.org/u/amandah)\ **Replies:** 0\ **Last updated:** [September 26, 2018, 6:43pm UTC](https://forum.allencell.org/t/culturing-cells-for-imaging-on-glass/32 "2018-09-26T18:43:36Z")
Growing our hiPSC lines on glass for imaging is a little bit different than growing them on plastic. We recommend phenol-red free Matrigel, and only coating plates at room temperature for 1 hour prior to seeding cells. … --- ## [When should I use ROCK inhibitor?](https://forum.allencell.org/t/when-should-i-use-rock-inhibitor/30)
**Author:** [@amandah](https://forum.allencell.org/u/amandah)\ **Replies:** 2\ **Last updated:** [December 7, 2019, 11:55pm UTC](https://forum.allencell.org/t/when-should-i-use-rock-inhibitor/30 "2019-12-07T23:55:33Z")
A common question we get is about when should media contain ROCK inhibitor (molecule Y-27632)? As a rule of thumb, cells should be fed media containing ROCK inhibitor 24 hours after thawing, and for 24 hours after each … --- ## [Single cell vs colony passaging](https://forum.allencell.org/t/single-cell-vs-colony-passaging/150)
**Author:** [@amulero6](https://forum.allencell.org/u/amulero6)\ **Replies:** 1\ **Last updated:** [December 7, 2019, 11:47pm UTC](https://forum.allencell.org/t/single-cell-vs-colony-passaging/150 "2019-12-07T23:47:50Z")
What is the advantage of single-cell passaging versus common colony passaging? Is there a desired confluency for single-cell passaging that leads to colony formation and vice-versa? Can you send pictures on how they shou… --- ## [Stem Cell Characteristic Markers](https://forum.allencell.org/t/stem-cell-characteristic-markers/119)
**Author:** [@kcordes](https://forum.allencell.org/u/kcordes)\ **Replies:** 0\ **Last updated:** [October 1, 2019, 9:07pm UTC](https://forum.allencell.org/t/stem-cell-characteristic-markers/119 "2019-10-01T21:07:00Z")
Are there any other biomarkers done on each of the cell lines available in the Cell Catalog besides the ones mentioned under the Stem Cell characteristics? (For example, other than NANOG and SOX-2) --- ## [Matrigel dilutions](https://forum.allencell.org/t/matrigel-dilutions/31)
**Author:** [@amandah](https://forum.allencell.org/u/amandah)\ **Replies:** 0\ **Last updated:** [September 26, 2018, 6:41pm UTC](https://forum.allencell.org/t/matrigel-dilutions/31 "2018-09-26T18:41:12Z")
We take a conservative approach to diluting our Matrigel because of morphology differences we see when our hiPSC are plated on glass. We observe the best morphology on glass with a 1:30 dilution of Matrigel, and our cell… --- ## [Pen/Strep or no Pen/Strep](https://forum.allencell.org/t/pen-strep-or-no-pen-strep/91)
**Author:** [@amandah](https://forum.allencell.org/u/amandah)\ **Replies:** 1\ **Last updated:** [August 6, 2019, 2:30pm UTC](https://forum.allencell.org/t/pen-strep-or-no-pen-strep/91 "2019-08-06T14:30:55Z")
The Allen Institute protocols recommend growing the cells with 1% P/S, but that’s just a recommendation. We’re a production lab, so we’ve chosen to use P/S in our media, but if you don’t want to use it that should be co… --- ## [Have you guys began hiPSC differentiation in to cardiomyocytes?](https://forum.allencell.org/t/have-you-guys-began-hipsc-differentiation-in-to-cardiomyocytes/41)
**Author:** [@kim.cordes](https://forum.allencell.org/u/kim.cordes)\ **Replies:** 3\ **Last updated:** [September 27, 2018, 6:26pm UTC](https://forum.allencell.org/t/have-you-guys-began-hipsc-differentiation-in-to-cardiomyocytes/41 "2018-09-27T18:26:02Z")
Have you guys began hiPSC differentiation in to cardiomyocytes? --- ## [Differentiation to other cell types](https://forum.allencell.org/t/differentiation-to-other-cell-types/81)
**Author:** [@Joel\_Luethi](https://forum.allencell.org/u/Joel_Luethi)\ **Replies:** 1\ **Last updated:** [February 13, 2019, 9:51pm UTC](https://forum.allencell.org/t/differentiation-to-other-cell-types/81 "2019-02-13T21:51:48Z")
Have you differentiated these hiPSC cell lines into other cell types than cardiomyocytes? Can you recommend protocols for other differentiations with these cells? I would like to compare the differentiation into differen… [Previous page](https://forum.allencell.org/c/hipsc-culture/10.md)